ABSTRACT
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Subject(s)
Humans , Male , Female , Adult , Therapeutic Equivalency , Chromatography, High Pressure Liquid/instrumentation , Gemifloxacin/analysis , Gemifloxacin/pharmacokinetics , Plasma , Ultraviolet RaysABSTRACT
The purpose of this experimental study was to determine the diametric variations of a brand of handheld stainless-steel K-files, acquired from different countries, in accordance with the available standards. 20 Mani stainless-steel K-files of identical size [ISO#25] were acquired from Pakistan and were designated as Group A while 20 Mani K-files were purchased from London, UK and designated as Group B. Files were assessed using profile projector Nikon B 24V. Data was statistically compared with ISO 3630:1 and ADA 101 by one sample T test. Significant difference was found between Groups A and B. Average discrepancy of Group A fell within the tolerance limit while that of Group B exceeded the limit. Findings in this study call attention towards adherence to the dimensional standards of stainless-steel endodontic files
ABSTRACT
Objective of the study was to qualitatively determine and compare the fracture patterns of hand held stainless steel K files [Mani, Inc. Japan], acquired from Pakistan and United Kingdom. This study was conducted at the Institute of Space Technology, over a period of one month. A total of 40 stainless-steel K files [Mani, Inc. Japan] of identical size [ISO#25] were collected and divided into two groups, such that Group A consisted of 20 K files acquired from the Rawalpindi/ Islamabad region in Pakistan while Group B consisted of 20 K files that were purchased from London, UK. The files were fractured one by one, 3mm from the tip. The fractured surfaces were then observed under scanning electron microscope to determine their fracture patterns. Evaluation of the fractured surfaces revealed decreased ductility in Group A in comparison with Group B. Stainless steel endodontic files available commercially in Pakistan need evaluation of their fracture behavior for patient safety and benefit
ABSTRACT
Background: Backache is second most common problem presenting to the primary healthcare providers. Lumbar discectomy has been revolutionized from open conventional discectomy to endoscopic removal. Endoscopic procedures are proving their superiority regarding good outcome, less wound site pain and shorter hospital stay, in Neurosurgery as well. Micro discectomy and Endoscopic discectomy is used only in few centres in Pakistan. This study aimed to share our experience of early surgical outcome endoscopic lumbar discectomy in terms postoperative pain improvement and duration of hospital stay
Methods: This prospective study was carried out at Neurosurgery Department, Jinnah Hospital, Lahore from Jan 2014 to Jan 2016. During this period, 35 patients of both sexes, aging between 20 and 60 years, with symptoms and signs of lumbago with sciatica were enrolled. Data was collected on a questionnaire after informed verbal and written consent
Results: A total of 35 patients were operated including 10 males and 25 females. Their age ranged from 20 to 60 years with mean age 33.14+/-8 years. Majority [32, 91%] of the patients had left side prolapsed paracentral disc, and remaining [3, 9%] had right sided prolapsed disc. Regarding the level of disc 19 [54%] patients had L4- 5 while 15 [43%] had L5-S1 and remaining one [2.9%] patients had L3-4 level. The post-op wound site visual analogue score was 1.57+/-1.1. Twenty-five patients had VAS of 1 [71.4%]. Minimum hospital stay was 1 day in 16 [45.75%] patients and maximum was 4 days in 3 [8.6%] patients. Mean hospital stay was 1.83+/-0.95 days. The only complication encountered was iatrogenic dural tear seen in one patient but with no CSF leak from wound site. No surgical site infections were reported at follow-ups
Conclusion: Endoscopic Lumbar discectomy is a safe procedure with short hospital stay
ABSTRACT
A fast, accurate, sensitive, selective and reliable method using reversed-phase high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry was developed and validated for the determination of finasteride in human plasma. After protein precipitation with perchloric acid, satisfactory separation was achieved on a Zorbax Eclipse[registered] C[8] analytical column using a mobile phase consisted of acetonitrile, 2 mM ammonium formate buffer [58:42, pH adjusted at 2.5 using formic acid]; the flow rate was 0.25 mLmin[-1] and the column oven was set to 50°C. Tamoxifen citrate was used as internal standard. This method involved the use of [M + H][+] ions of ?nasteride and IS at m/z 373 and 372 respectively with the selected ion monitoring [SIM] mode. The calibration curve was linear over the range of 0.1-60 ng mL[-1]. The limit of quantification for finasteride in plasma was 0.1 ng mL[-1]. The intra-day and inter-day repeatability [precision] were 2.68-13.87% and 2.14-14.69% respectively. Intra-day and inter-day accuracy were 98-101.57% and 99.7-110%. The assay method has been successfully used to estimate the pharmacokinetics of finasteride after oral administration of a 5 mg tablet of finasteride in 12 healthy volunteers